plasmid verification
[PLAZ-mid ver-i-fi-KAY-shun]
nounpl: plasmid verifications
verificação de plasmídeo
1. A molecular biology process used to confirm the presence, identity, and integrity of a plasmid (circular DNA molecule) in bacterial cells or cloning vectors
Plasmid verification was performed using restriction enzyme digestion and gel electrophoresis to ensure the insert was correctly ligated.
A verificação de plasmídeo foi realizada usando digestão com enzimas de restrição e eletroforese em gel para garantir que o inserto foi ligado corretamente.
2. The collection of laboratory techniques and assays used to validate plasmid structure, copy number, and genetic content
After transformation, plasmid verification is essential before proceeding with protein expression experiments.
Após a transformação, a verificação de plasmídeo é essencial antes de prosseguir com os experimentos de expressão proteica.
Plasmid verification is a standard procedure in biotechnology and molecular biology research in both Brazil and the USA. In Brazilian universities and research institutes (CNPq, FAPESP-funded laboratories), this term is integral to genetic engineering curricula. The process reflects the globalized nature of scientific terminology, with Portuguese-speaking scientists using largely similar methodologies as their English-speaking counterparts.
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