plasmid confirmation
[PLAZ-mid kun-fur-MAY-shun]
nounpl: plasmid confirmations
confirmação de plasmídeo
1. A molecular biology procedure used to verify the presence, identity, and integrity of a plasmid in a bacterial or yeast cell, typically through DNA sequencing, restriction enzyme digestion, or PCR analysis
The researcher performed plasmid confirmation using restriction enzyme digestion to ensure the insert was correctly integrated.
O pesquisador realizou a confirmação do plasmídeo usando digestão com enzimas de restrição para garantir que o inserto foi integrado corretamente.
2. Laboratory validation that a cloned or transformed plasmid contains the desired DNA sequence and is present in the target host organism
Plasmid confirmation is an essential step before proceeding with protein expression experiments.
A confirmação do plasmídeo é uma etapa essencial antes de prosseguir com experimentos de expressão de proteína.
This is specialized scientific terminology used primarily in molecular biology laboratories in Brazil, Portugal, and internationally. The term is essential in genetic engineering and biotechnology research contexts. In Brazilian universities and research institutions (FAPESP-funded labs, for example), English terminology is often used interchangeably with Portuguese translations during technical discussions and publications.
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