restriction nuclease
[/rɪˈstrɪkʃən ˈnjuːkliːeɪz/]
nounpl: restriction nucleases
endonuclease de restrição
1. An enzyme that cuts DNA at specific recognition sequences, commonly used in molecular biology and genetic engineering
The restriction nuclease EcoRI recognizes the palindromic sequence GAATTC and cuts the DNA strand at that location.
A endonuclease de restrição EcoRI reconhece a sequência palindrômica GAATTC e corta a fita de DNA nesse local.
2. A bacterial enzyme that acts as a molecular scissors to cleave double-stranded DNA molecules
Scientists use restriction nucleases to create recombinant DNA by cutting and combining DNA fragments from different organisms.
Os cientistas usam endonucleases de restrição para criar DNA recombinante cortando e combinando fragmentos de DNA de diferentes organismos.
This is a highly specialized scientific term predominantly used in molecular biology, biotechnology, and genetic engineering fields. It originated with the discovery of bacterial restriction-modification systems and has become fundamental to modern molecular biology research and gene therapy applications. The term is consistently used across English and Portuguese-speaking scientific communities with minimal regional variation.
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