RT-qPCR
[ɑr ti kjuː piː siː ɑr]
noun
RT-qPCR
1. A molecular biology technique that combines reverse transcription with quantitative polymerase chain reaction to detect and quantify RNA molecules in real-time
RT-qPCR is commonly used to measure gene expression levels in cells.
RT-qPCR é comumente utilizado para medir os níveis de expressão gênica em células.
2. An acronym for Reverse Transcription quantitative Polymerase Chain Reaction, also known as real-time RT-PCR
The researchers performed RT-qPCR to validate their RNA-sequencing results.
Os pesquisadores realizaram RT-qPCR para validar seus resultados de sequenciamento de RNA.
RT-qPCR is a standardized molecular biology technique used globally in research institutions, diagnostic laboratories, and quality control settings. In Brazil and Portugal, it is primarily used in academic research, pharmaceutical development, and clinical diagnostics. The term is not translated but maintained as an acronym in both Portuguese-speaking countries, reflecting the international standardization of scientific nomenclature. During the COVID-19 pandemic, RT-qPCR gained prominence as the gold standard for SARS-CoV-2 detection in both Brazil and Portugal.
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