DNA cloning
[/ˌdiː.ɛnˈeɪ ˈkloʊnɪŋ/]
noun
clonagem de DNA
1. The process of creating an identical copy of a DNA molecule or organism by using recombinant DNA technology
DNA cloning is essential for producing recombinant proteins in genetic engineering.
A clonagem de DNA é essencial para a produção de proteínas recombinantes em engenharia genética.
2. A molecular biology technique that involves inserting a DNA fragment into a vector to replicate and produce multiple copies
The researchers used DNA cloning to amplify the target gene sequence.
Os pesquisadores utilizaram clonagem de DNA para amplificar a sequência de genes alvo.
3. The creation of genetically identical organisms or cells derived from a single parent
DNA cloning techniques have made it possible to create transgenic animals.
As técnicas de clonagem de DNA tornaram possível criar animais transgênicos.
DNA cloning is a cornerstone technique in modern biotechnology and molecular biology, widely taught in universities and used in pharmaceutical research across both Brazil and the USA. The term is used identically in both Portuguese-speaking regions and English-speaking countries, reflecting the international nature of scientific terminology. In Brazil, this technique is particularly important in agricultural biotechnology and medical research institutions.
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